30 INTEGRANTES

GRUPLAC

DESCRIPCIÓN 

El grupo de investigación tiene como objetivo generar conocimiento en áreas relevantes para el país en temáticas relacionadas con la salud y producción animal, medicina de poblaciones, zoonosis y medicina de la conservación. Para el cumplimiento de este objetivo el grupo desarrolla proyectos de investigación enmarcado en cinco líneas de investigación las cuales son: Producción animal, Medicina de Poblaciones, Medicina de la Conservación, Zoonosis y Salud Animal. Los proyectos realizados en el campo de la medicina de la conservación han permitido la conservación de especies nativas tales como el pez capitán de la sabana y la Pacarana.

PRODUCTOS DESTACADOS

Using of Okara in diets for growing broilers

This study aimed to evaluate the effects of okara inclusion in diet for growing broilers on performance, carcass yield, blood and bone variables, quality and lipid oxidation of meat and economic viability. For that, 575 Cobb 21-days-old male broilers were distributed in a completely randomized design with four levels of okara inclusion (25, 50, 75 and 100 g of okara/kg diet) and a control group with five replicates and 23 birds each. There was no difference (P>0.05) in function of okara levels on the performance variables, carcass yield, bone variables and serum triglycerides, calcium and phosphorus at 42 days old. Serum cholesterol levels showed a quadratic response (P<0.05), in which the lowest value estimated was 65.3 g of okara/kg of diet. Okara can be included in diets for broilers up to the level of 100g/kg without affecting the performance, carcass yield, bone variables and lipid oxidation of meat. However, the best economic results were observed up to 50g of okara/kg of diet.

Resposta de linfonodos em bovinos inoculados a campo com a vacina recombinante rSBm7462 anti Rhipicephalus (Boophilus) microplus)

A dependência exclusiva de compostos químicos para o controle de Rhipicephalus (Boophilus) microplus tornou-se uma das maiores preocupações científicas e econômicas dos últimos anos, e como consequência, estão sendo realizadas pesquisas para o desenvolvimento de vacinas. O objetivo deste trabalho foi avaliar a resposta de linfonodos de bovinos imunizados a campo com o peptídeo rSBm7462 anti R. (B.) microplus. Foram utilizados 14 bovinos mestiços (Bos taurus x Bos indicus), com idades entre 4-10 meses, mantidos em duas propriedades rurais do norte do estado de Minas Gerais. Os animais receberam três imunizações do peptídeo rSBm7462, aplicados por via subcutânea, com intervalo de 30 dias. Após 15 dias de cada imunização, os linfonodos pré-escapulares foram coletados e fixados por 18 horas em formol. Posteriormente, foram incluídos em Paraplast e as amostras foram coradas pela técnica hematoxilina-eosina (HE) para a observação de eventos celulares. Para a identificação do antígeno nos linfonodos dos animais imunizados, foi realizada a técnica de imuno-histoquímica (IHQ) com o método peroxidase-anti-peroxidase (PAP). A resposta de linfonodos dos bovinos inoculados foi avaliada pelas análises de formação de centros germinais (CG), hiperplasia de cordões medulares (CM) e a presença do antígeno rSBm7462 em células PAP+, demonstrando que o peptídeo recombinante rSBm7462 induz uma resposta imune adaptativa T-dependente, caracterizada nos tecidos linfóides secundários pela formação de estruturas que conferem afinidade e memória imunológica.

Plasmodium vivax in vitro continuous culture: The spoke in the wheel

Understanding the life cycle of Plasmodium vivax is fundamental for developing strategies aimed at controlling and eliminating this parasitic species. Although advances in omic sciences and high-throughput techniques in recent years have enabled the identification and characterization of proteins which might be participating in P. vivax invasion of target cells, exclusive parasite tropism for invading reticulocytes has become the main obstacle in maintaining a continuous culture for this species. Such advance that would help in defining each parasite protein’s function in the complex process of P. vivax invasion, in addition to evaluating new therapeutic agents, is still a dream. Advances related to maintenance, culture medium supplements and the use of different sources of reticulocytes and parasites (strains and isolates) have been made regarding the development of an in vitro culture for P. vivax; however, only some cultures having few replication cycles have been obtained to date, meaning that this parasite’s maintenance goes beyond the technical components involved. Although it is still not yet clear which molecular mechanisms P. vivax prefers for invading young CD71+ reticulocytes [early maturation stages (I-II-III)], changes related to membrane proteins remodelling of such cells could form part of the explanation. The most relevant aspects regarding P. vivax in vitro culture and host cell characteristics have been analysed in this review to explain possible reasons why the species’ continuous in vitro culture is so difficult to standardize. Some alternatives for P. vivax in vitro culture have also been described

Ovine and caprine fasciolosis incidence in the north of Cesar and south of The Guajira. Incidencia de Fasciolosis Ovina y Caprina En El Norte Del Cesar y Sur de La Guajira.

La fasciolosis es una enfermedad importante causada por el parásito Fasciola hepática que generalmente acomete a animales de producción especialmente rumiantes domésticos y ocasionalmente al hombre por lo cual tiene una gran importancia en salud pública. Descrita comúnmente en regiones templadas y húmedas, el transporte indiscriminado de animales ha permitido que este tremátodo se asiente y complete el ciclo biológico en regiones del trópico seco, como el norte del Cesar y sur de La Guajira de Colombia. Objetivo: identificar la incidencia de fasciolosis en ovinos y caprinos. Métodos: 1039 ovinos y caprinos seleccionados aleatoriamente sin discriminación de sexo o edad de diez municipios de los dos departamentos. Se colectaron muestras de materia fecal por palpación rectal (apróx. 100 g para diagnóstico parasitológico) mediante la técnica de Dennis a partir de muestras colectadas en agosto del 2015 a mayo del 2016, los huevos recuperados fueron analizados morfológicamente a través de microscopía de luz (aumentos de 10 x y 40 x). Resultados: para el total de la población analizada de 1039 animales de las dos especies, 150 se encontraron positivos (14,43%), un total de 27 en caprinos (18%): 26 hembras y 1 macho; 123 en ovinos (82%), 111 hembras y 12 machos. Conclusiones: se demuestra con este estudio la importancia de fasciolosis en la ganadería ovino-caprina del norte del Cesar y sur de la Guajira para que se tomen medidas de control en procesos de inspección sanitaria por su impacto en salud pública.

On the evolution and function of Plasmodium vivax reticulocyte binding surface antigen (pvrbsa)

The RBSA protein is encoded by a gene described in Plasmodium species having tropism for reticulocytes. Since this protein is antigenic in natural infections and can bind to target cells, it has been proposed as a potential candidate for an anti-Plasmodium vivax vaccine. However, genetic diversity (a challenge which must be overcome for ensuring fully effective vaccine design) has not been described at this locus. Likewise, the minimum regions mediating specific parasite-host interaction have not been determined. This is why the rbsa gene’s evolutionary history is being here described, as well as the P. vivax rbsa (pvrbsa) genetic diversity and the specific regions mediating parasite adhesion to reticulocytes. Unlike what has previously been reported, rbsa was also present in several parasite species belonging to the monkey-malaria clade; paralogs were also found in Plasmodium parasites invading reticulocytes. The pvrbsa locus had less diversity than other merozoite surface proteins where natural selection and recombination were the main evolutionary forces involved in causing the observed polymorphism. The N-terminal end (PvRBSA-A) was conserved and under functional constraint; consequently, it was expressed as recombinant protein for binding assays. This protein fragment bound to reticulocytes whilst the C-terminus, included in recombinant PvRBSA-B (which was not under functional constraint), did not. Interestingly, two PvRBSA-A-derived peptides were able to inhibit protein binding to reticulocytes. Specific conserved and functionally important peptides within PvRBSA-A could thus be considered when designing a fully-effective vaccine against P. vivax. © 2018 Camargo-Ayala, Garzón-Ospina, Moreno-Pérez, Ricaurte-Contreras, Noya and Patarroyo.

GALERÍA