Las líneas de trabajo del GRUPO INVESTIGACIONES BIOMÉDICAS Y DE GENÉTICA HUMANA APLICADA GIBGA buscan generar investigación biomédica que cubra varios frentes importantes a nivel nacional.

Es el objetivo desarrollar estudios sobre:

  • las correlaciones de la estructura metabólica, la virulencia y la filogeográfica de cepas de Helicobacter pylori para la identificación de blancos terapéuticos.
  • Problemas concernientes a la fisiología y anatomía comparada humana.
  • La determinación de la genotoxicidad en muestras de personal en empleos que se encuentren en alto riesgo de exposición a genotóxicos y cancerígenos.
  • La creación de un Laboratorio de diagnóstico molecular del cáncer e identificación de genes que confieran susceptibilidad a los mismos.
  • Establecimiento de líneas celulares de cáncer de estomago, mama y pulmón e identificación de metabolitos inhibidores del crecimiento.

Nuestra orientación trata de potenciar la conexión entre la investigación básica, clínica, epidemiológica y computacional sobre temas pertinentes a nuestra región.



Helicobacter pylori in water sources and food products: A constant public health problem

Helicobacter pylori is a curved, microaerophilic, multi-flaked, Gram-negative bacillus associated with different gastrointestinal disorders. It´s still a relevant issue in public health due to several scientific investigations that are being carried out in different subjects as, virulence factors, ecosystem interactions, immune response, eradication strategies and Pathogen Transmission Routes in food and water sources. The present review included the association between food, water sources and H. pylori, also, the importance of promoting preventive measures for this pathogen transmission, such as the improvement of the living conditions of families and good hygienic practices in the preparation of food, to prevent the infection by this pathogen, considering, that food and drinking water has always played a very important role as vectors of this pathogen. © 2019, SILAE (Italo-Latin American Society of Ethnomedicine). All rights reserved.

Apparent origin of glossopharyngeal, vagus and accessory nerves: An aspect to consider in human neuroanatomy teaching

In various neuroanatomy texts and articles related to this area of knowledge, there is a conceptual vacuum associated with the precise sites where the roots of the cranial nerves emerge. The objective of the study was to establish the exact location of the apparent origin of the glossopharyngeal, vagus and accessory cranial nerves in the medulla oblongata of the human being 120 human brainstems, previously fixed in formalin solution at 10 % were assessed, the location where such nerve roots emerge was identified by direct examination and once the piamater was removed at both right and left sides as it has been stated in the literature. It was found that in 100 % of the studied brainstems their nerve roots emerge on average at about 2.63 mm behind the retro-olivary groove, different to what has been stated in the literature. Glossopharyngeal, vagus and accessory human nerves do not emerge directly from the retro-olivary groove, as commonly reported; instead, they emerge behind the said groove, specifically in the retro-olivary groove area, where they form a continuous line of nerve roots. © 2019, Universidad de la Frontera. All rights reserved.

Antiproliferative activity of extracts of Gnaphalium Gracile H.B.K. against cancer cell lines

Ethanol and n-hexane extracts obtained from the leaves and inflorescences of Gnaphalium gracile, were tested at different concentrations to evaluate their antineoplastic activities on pancreatic, colon, and prostate cancer cell lines by examining mitochondrial function. The polar extracts of both, leaves and inflorescences which contain gnaphalin, quercetin, and 3-methoxy quercetin, exhibited cytotoxicity against every cell line tested with EC50 values ranging between 20.23±1.185 µg/mL and 70.71±1.1419 µg/mL. The most remarkable values were observed in pancreatic cancer Panc 28 and androgen-dependent prostate LnCaP cells, with EC50 values of 20.23±1.185 and ˂25µg/mL, and androgen-independent prostate cancer PC-3, colon HCT-116 and pancreatic MIA PaCa cells with values ranging between 28.84±1.1766 and 34.41±1.057 µg/mL. The non-polar extract derived from leaves demonstrated significant cytotoxicity towards colon cancer HCT-116 cells, with an EC50 of 39.46±1.0617 µg/mL. However, the non-polar extract from the inflorescences did not have an appreciable effect on cell proliferation of any of the cell lines tested except for androgen-independent prostate cancer PC-3 cells with an EC50 of 62.05±1.237 µg/mL. The data obtained support the traditional use of G. gracile and suggest the polar extracts from aerial parts, as an interesting source for the development of novel antineoplastic agents. © 2018, SILAE (Italo-Latin American Society of Ethnomedicine). All rights reserved.

Antiproliferative activity of chloroformic fractions from leaves and inflorescences of Ageratina gracilis

To obtain a scientific basis and justification of plant domestication in the use of Ageratina gracilis, we did an in vitro study of the anticancer potential of extracts and fractions from its leaves and inflorescences. Firstly, cytotoxicity was evaluated against five human tumorigenic cell lines by MTT assay. Subsequently, the chloroformic fractions, considered the most cytotoxic were tested for genotoxicity by comet assay, morphological effects were analyzed by fluorescent microscopy, cell cycle arrest by flow cytometry and early apoptosis induction through fluorescein-5-isothiocyanate (FITC) labeled Annexin-V assay. Non-polar extracts with IC50 values of <53μg/ml showed a high cytotoxicity. The highest cytotoxicity was achieved by chloroformic fraction from petroleum ether extract of leaves and inflorescences and chloroformic fraction from ethanolic extract of leaves, displaying a significant inhibition of cell viability particularly on A549 cells with an IC50 value of 25.9 μg/mL. Chloroformic fractions caused a high percent of DNA damage above 60 percent on A549 and MDAMB-231.The fractions also induced G1/S phase arrest of the cell cycle in A549 cells, furthermore it was confirmed the apoptotic activity chloroformic fraction from petroleum ether extract of inflorescences and chloroformic fraction from ethanolic extract of leaves on those cells by Annexin-V assay. These preliminary results indicate that A. gracilis has an antiproliferative activity against cancer cells, being a starting point for forthcoming studies about the antineoplastic activity and its domestication conditions.