Gina Marcela Méndez Callejas

 gmendez@udca.edu.co

LÍNEAS DE INVESTIGACIÓN:   Biología Celular y Molecular del Cáncer

FACULTAD:  Ciencias de la Salud

CATEGORÍA COLCIENCIAS:    Senior

NIVEL DE FORMACIÓN: Doctorado

Doctora en Ciencias Biología, de la Università degli Studi Roma Tre, Italia, Magister en Biología de la Universidad Javeriana y Licenciada en Química de la Universidad Distrital Francisco José de Caldas. Con amplia experiencia en cátedra universitaria en las asignaturas de Bioquímica, Biología Celular, Química General, e Inmunología para programas de Ciencias Naturales e Ingenierías y Ciencias de Salud. Con trayectoria y especial interés en proyectos de investigación enfocados hacia la expresión y regulación de blancos moleculares de agentes con potencial quimioterapéutico de origen natural y sintético. Entre los trabajos relevantes se encuentran la validación experimental de la red de interacción asociada a la expresión de proteínas pro-apoptóticas así como el estudio de las funciones y la regulación de proteínas implicadas en muerte celular inducida por compuestos polifenólicos de especies del género Chromolaena, consideradas como plantas medicinales con potencial antioxidante y antiproliferativo. Los diferentes proyectos han permitido adquirir habilidades en técnicas inmunológicas, de Biología Celular, Molecular y Microscopía, Técnicas cromatográficas para la separación, purificación e identificación de proteínas implicadas en mecanismos de acción de moléculas con potencial terapéutico en cáncer.

PRODUCTOS DESTACADOS

Topological properties and in vitro identification of essential nodes of the Paclitaxel and Vincristine interactomes in PC-3 cells
Fecha de publicación: 31/10/2019

https://www.sciencedirect.com/science/article/pii/S2319417018302580


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Cryptosporidium spp. CP15 and CSL protein-derived synthetic peptides’ immunogenicity and in vitro seroneutralisation capability
Fecha de publicación: 29/10/2018

Cryptosporidium spp. is a zoonotic intracellular protozoan and a significant cause of diarrhoea in humans and animals worldwide. This parasite can cause high morbidity in immunocompromised people and children in developing countries, livestock being the main reservoir. This study was aimed at performing preliminary tests on Swiss albino weaned mice (ICR) to evaluate the humoral immune response induced against peptides derived from Cryptosporidium parvum CP15 (15 kDa sporozoite surface antigen) and CSL (circumsporozoite-like antigen) proteins. Peptides were identified and characterised using bioinformatics tools and were chemically synthesised. The antibody response was determined and the neutralising effect of antibodies was measured in cell culture. Despite all peptides studied here were capable of stimulating antibody production, neutralising antibodies were detected for just two of the CP15-derived ones. Additional studies aimed at evaluating further the potential of such peptides as vaccine candidates are thus recommended. © 2018 Elsevier Ltd


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Antiproliferative activity of chloroformic fractions from leaves and inflorescences of Ageratina gracilis
Fecha de publicación: 01/01/2017

To obtain a scientific basis and justification of plant domestication in the use of Ageratina gracilis, we did an in vitro study of the anticancer potential of extracts and fractions from its leaves and inflorescences. Firstly, cytotoxicity was evaluated against five human tumorigenic cell lines by MTT assay. Subsequently, the chloroformic fractions, considered the most cytotoxic were tested for genotoxicity by comet assay, morphological effects were analyzed by fluorescent microscopy, cell cycle arrest by flow cytometry and early apoptosis induction through fluorescein-5-isothiocyanate (FITC) labeled Annexin-V assay. Non-polar extracts with IC50 values of <53μg/ml showed a high cytotoxicity. The highest cytotoxicity was achieved by chloroformic fraction from petroleum ether extract of leaves and inflorescences and chloroformic fraction from ethanolic extract of leaves, displaying a significant inhibition of cell viability particularly on A549 cells with an IC50 value of 25.9 μg/mL. Chloroformic fractions caused a high percent of DNA damage above 60 percent on A549 and MDAMB-231.The fractions also induced G1/S phase arrest of the cell cycle in A549 cells, furthermore it was confirmed the apoptotic activity chloroformic fraction from petroleum ether extract of inflorescences and chloroformic fraction from ethanolic extract of leaves on those cells by Annexin-V assay. These preliminary results indicate that A. gracilis has an antiproliferative activity against cancer cells, being a starting point for forthcoming studies about the antineoplastic activity and its domestication conditions.


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