Diego Vicente Moreno Rodríguez

LÍNEAS DE INVESTIGACIÓN:   Salud Humana y Animal y Sostenibilidad Ambiental


PROGRAMA:  Química



Doctor en Química por el CINVESTAV (México), Magister en Ciencias Química por la Universidad Nacional de Colombia (Bogotá), Químico de la Universidad Nacional de Colombia (Bogotá). Tengo mucho interés en el desarrollo de herramientas computacionales orientadas a la Química, Nos encontramos creando una nueva herramienta en Química Computacional que permitirá estudiar reacciones de forma extensiva.  Tengo experiencia en el desarrollo e implementación de herramientas computacionales en estructura electrónica, además de la investigación y búsqueda de nuevos compuestos o materiales de interés. También en la creación de nuevas herramientas para entender el enlace Químico. Investigación empleando herramientas de metagenómica y Diseño de fármacos Asistido por Computadora.


LINEAS DE TRABAJO:   Química Computacional, Química Cuántica, Química Teórica


Transcriptome analysis reveals novel insights into the response of low-dose benzo (a) pyrene exposure in male tilapia
Fecha de publicación: 01/08/2018

Despite a wide number of toxicological studies that describe benzo[a]pyrene (BaP) effects, the metabolic mechanisms that underlie these effects in fish are largely unknown. Of great concern is the presence of BaP in aquatic systems, especially those in close proximity to human activity leading to consumption of potentially contaminated foods. BaP is a known carcinogen and it has been reported to have adverse effects on the survival, development and reproduction of fish. The purpose of this study was to investigate if a low dose of BaP can alter genes and key metabolic pathways in the liver and testis in male adult tilapia, and whether these could be associated with biological endpoints disruption. We used both high-throughput RNA-Sequencing to assess whole genome gene expression following repeated intraperitoneal injections of 3 mg/kg of BaP (every 6 days for 26 days) and morphometric endpoints as indicators of general health. Condition factor (K) along with hepatosomatic and gonadosomatic indices (morphometric parameters) were significantly lower in BaP-treated fish than in controls. BaP exposure induced important changes in the gene expression pattern in liver and testis as revealed by both Pathway and Gene Ontology (GO) analyses. Alterations that were shared by both tissues included arachidonic acid metabolism, androgen receptor to prostate-specific antigen signaling, and insulin-associated effects on lipogenesis. The most salient liver-specific effects included: biological processes involved in detoxification, IL6-associated insulin resistance, mTOR hyperactivation, mitotic cytokinesis, spindle pole and microtubule binding. BaP effects that were confined to the testis included: immune system functions, inflammatory response, estrogen and androgen metabolic pathways. Taken together, gene expression and morphometric end point data indicate that the reproductive success of adult male tilapia could be compromised as a result of BaP exposure. These results constitute new insights on the mechanism of action of low dose BaP in a non-model organism (tilapia).

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