Biólogo con Maestría y Doctorado en el área de las Ciencias Biomédicas y Biológicas. Cuenta con experiencia en Docencia Universitaria de 4 años, impartiendo las áreas de Biología Celular y Molecular, Parasitología General, Biotecnología e Investigación y en la formación de Recurso Humano a través de la dirección de Diplomados, Pasantías, Iniciación Científica (Joven Investigador), Pregrados y Maestrías. Ha laborado por 10 años en Investigación Básica donde formuló, coordinó y desarrolló Proyectos de Investigación con énfasis en Biología Molecular, Celular e Inmunoquímica y publicó 31 artículos en revistas nacionales (2) e internacionales (29).
LINEAS DE TRABAJO: Parasitología, Biología Molecular, Identificación de Antígenos
PRODUCTOS DESTACADOS
Babesia Bovis Ligand-Receptor Interaction: AMA-1 Contains Small Regions Governing Bovine Erythrocyte Binding
Fecha de publicación: 13/01/2021
Apical membrane antigen 1 is a microneme protein which plays an indispensable role during Apicomplexa parasite invasion. The detailed mechanism of AMA-1 molecular interaction with its receptor on bovine erythrocytes has not been completely defined in Babesia bovis. This study was focused on identifying the minimum B. bovis AMA-1-derived regions governing specific and high-affinity binding to its target cells. Different approaches were used for detecting ama-1 locus genetic variability and natural selection signatures. The binding properties of twelve highly conserved 20-residue-long peptides were evaluated using a sensitive and specific binding assay based on radio-iodination. B. bovis AMA-1 ectodomain structure was modelled and refined using molecular modelling software. NetMHCIIpan software was used for calculating B- and T-cell epitopes. The B. bovis ama-1 gene had regions under functional constraint, having the highest negative selective pressure intensity in the Domain I encoding region. Interestingly, B. bovis AMA-1-DI (100YMQKFDIPRNHGSGIYVDLG119 and 120GYESVGSKSYRMPVGKCPVV139) and DII (302CPMHPVRDAIFGKWSGGSCV321)-derived peptides had high specificity interaction with erythrocytes and bound to a chymotrypsin and neuraminidase-treatment sensitive receptor. DI-derived peptides appear to be exposed on the protein’s surface and contain predicted B- and T-cell epitopes. These findings provide data (for the first-time) concerning B. bovis AMA-1 functional subunits which are important for establishing receptor-ligand interactions which could be used in synthetic vaccine development.
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