Andrea Carolina Chiappe Pulido

GRUPOS DE INVESTIGACIÓN: PRODUCTOS NATURALES U.D.C.A

LÍNEAS DE INVESTIGACIÓN:   Sostenibilidad Ambiental

 

PROGRAMA: 

CATEGORÍA MINCIENCIAS:   

NIVEL DE FORMACIÓN: 

Licenciada en Química de la Universidad Distrital Francisco José de Caldas, mágister en Ciencias-Química de la Universidad Nacional de Colombia y estudiante de doctorado en Biología en la Universidad Humboldt de Berlin. Docente investigadora en las áreas de Biología molecular, Química orgánica y Productos naturales derivados de plantas.

LINEAS DE TRABAJO:   

PRODUCTOS DESTACADOS

Two isoforms of Arabidopsis protoporphyrinogen oxidase localize in different plastidal membranes
Fecha de publicación: 01/06/2023

All land plants encode 2 isoforms of protoporphyrinogen oxidase (PPO). While PPO1 is predominantly expressed in green tissues and its loss is seedling-lethal in Arabidopsis (Arabidopsis thaliana), the effects of PPO2 deficiency have not been investigated in detail. We identified 2 ppo2 T-DNA insertion mutants from publicly available collections, one of which (ppo2-2) is a knock-out mutant. While the loss of PPO2 did not result in any obvious phenotype, substantial changes in PPO activity were measured in etiolated and root tissues. However, ppo1 ppo2 double mutants were embryo-lethal. To shed light on possible functional differences between the 2 isoforms, PPO2 was overexpressed in the ppo1 background. Although the ppo1 phenotype was partially complemented, even strong overexpression of PPO2 was unable to fully compensate for the loss of PPO1. Analysis of subcellular localization revealed that PPO2 is found exclusively in chloroplast envelopes, while PPO1 accumulates in thylakoid membranes. Mitochondrial localization of PPO2 in Arabidopsis was ruled out. Since Arabidopsis PPO2 does not encode a cleavable transit peptide, integration of the protein into the chloroplast envelope must make use of a noncanonical import route. However, when a chloroplast transit peptide was fused to the N-terminus of PPO2, the enzyme was detected predominantly in thylakoid membranes and was able to fully complement ppo1. Thus, the 2 PPO isoforms in Arabidopsis are functionally equivalent but spatially separated. Their distinctive localizations within plastids thus enable the synthesis of discrete subpools of the PPO product protoporphyrin IX, which may serve different cellular needs.


Más información

investigaciones_pie_pagina